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Analysis of Transcriptional Response and Tolerance to Furfural in Industrial Yeast

Ying-jin Yuan, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China

To overcome various bottlenecks and achieve key technological breakthroughs to provide technical support for the economic production of cellulose based biofuels. We employ systems biology tools to elucidate the microbial capability of growing and producing bioethanol under extreme or stressed conditions, to reveal various molecular and biochemical mechanisms underlying its tolerance. The response to furfural in exponential phase of both wild industrial budding yeast and the tolerant strain was characterized by measuring transcript levels of the genome on a global genomic cDNA microarray. It was found that 652 genes and 121 genes were regulated more than twofold in response to furfural in wild industrial budding yeast and the tolerant strain, respectively. Comparing the two strains, we found that the difference in energy metabolism and stress response might contribute to the furfural tolerance.