Conductive Nanofibrous Composite Scaffolds Based on Polyaniline Nanoparticle and Polylactide for Bone Regeneration

Mimicking the nanofibrous structure similar to extracellular matrix and conductivity for electrical propagation would be highly beneficial for tissue engineering. Herein, we prepared conductive and nanofibrous scaffold via varying contents of PANI was in-situ polymerized on PLA electrospun nanofibers treated by plasma and dopamine-loaded to make the nanofibrous scaffold for in vitro hMSCs differentiation studies under electrical stimulation. It showed that the PLA/PANI conductive nanofibrous scaffold exhibited superior cell adhesion, growth and proliferation to pure PLA. Furthermore, the addition of conductive PANI to the 3D composite nanofibrous scaffold considerably enhanced hMSCs differentiation , osteogenic related gene expression and osteoinduction. Therefore the PLA/PANI conductive nanofibrous scaffold indicated promising potential applications in tissue engineering.