478827 Dynamic Tissue Culture Model for Ex Vivo Recreation of the Mechanical Microenvironment of the Lung

Monday, November 14, 2016
Grand Ballroom B (Hilton San Francisco Union Square)
Alexus Rockward1, Ning Ge2, David Heidary3, Chris Richards3 and Christine Trinkle2, (1)University of Kentucky, Lexington, KY, (2)Mechanical Engineering, University of Kentucky, Lexington, KY, (3)Chemistry, University of Kentucky, Lexington, KY

The purpose of the research is to create a lung-on-a-chip device that simulates the mechanical microenvironment. Traditional cell culture methods fail to account for the effects of mechanical factors, such as strain and pressure, on lung cells. Because lung cells respond to mechanical factors as well as biochemical factors, a failure to introduce mechanical factors in the microenvironment of the cells results in inaccurate or incomplete results. The goal of this research is to create a dynamic model for cell culture and assess its performance when compared to traditional cell culture methods. The dynamic model provides researchers with critical information that the traditional culture method cannot supply, such as the behavior of a mechanosensitive protein under in vivo conditions. This new model will be used to study mechanosensitive channel defects that cause cystic fibrosis.

Research Supported by the National Science Foundation REU Program #EED-1460486

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