478616 Determination of the Best Conditions of Biodiesel Production with Free Form Lipases on Refining Palm Oil to Laboratory Scale

Monday, November 14, 2016
Grand Ballroom B (Hilton San Francisco Union Square)
Jorge A. Graciano Ramírez, Facultad Salud, Institución Universitaria Colegio Mayor de Antioquia, Medellin, Colombia, Angela A. Ruiz Colorado, Procesos y Energia, Facultad de Minas, Universidad Nacional de Colombia, Medellin, Colombia and John H Castro, Facultad de Minas, Universidad Nacional de Colombia, Medellín, Colombia

The enzymatic production of biodiesel has different opportunities and advantages over chemical production as improvements in the production process of biodiesel and removing byproducts, treatment of different raw materials, reduced costs associated with the operation 1,2,3.However the production process of biodiesel with enzymes in free form, must overcome different constraints by conditions in the medium as the stability of reaction, yields, the possibility of reuse or costs associated with the reaction3. This project was aimed to find the best conditions that would allow a high conversion to biodiesel in the laboratory, which was performed by enzymatic catalysis RBD palm oil with the formulation Eversa Transform feeding regularly with methanol to a reaction period of 9 hours. The best conditions biodiesel production were molar ratio of alcohol - oil 4.1, 34 ° C and percentage of enzyme 1.1% for a 74% with a achieved prediction by the model of 80%, where finally the biodiesel convertion is affected with increased alcohol in the medium and decreasing temperature, and linearly favored by amount of enzyme. These conditions were found by implementing the methodology MSR.

KEYWORDS: Biodiesel, Palm Oil, Lipases, Methanol


  1. Shweta Shah, Shweta Sharma and MN Gupta. Enzymatic transesterification for Biodiésel production. Indian Journal of Biochemistry & Biophysics. 2003; Vol. 40. 392 - 399.
  2. Poppe, J. K., Fernandez-Lafuente, R., Rodrigues, R. C., & Ayub, M. A. Z. Enzymatic reactors for biodiésel synthesis: Present status and future prospects. Biotechnology Advances. 2015 33(5), 511–525. http://doi.org/http://dx.doi.org/10.1016/j.biotechadv.2015.01.011
  3. Fjerbaek, L., Christensen, K. V., & Norddahl, B. A review of the current state of biodiésel production using enzymatic transesterification. Biotechnology and Bioengineering. 2009. 102(5), 1298–1315.


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