Enzymatic CO2 Utilization Using Immobilized and Stabilized Carbonic Anhydrase

Carbonic anhydrase (CA) is a promising biocatalyst that can be used for the CO₂ conversion technologies. CA catalyzes the hydration of carbon dioxide (CO₂) to bicarbonate (HCO₃^-) at high turnover number. Due to its fast kinetics, the introduction of CA in CO₂ capture process can lead to various positive results including the reduction of reactor size as well as lowering the energy input at CO₂ stripper. However, the practical application of CA has been limited by its poor stability. This presentation will introduce nanobiocatalytic approach that can immobilize and stabilize CA using various nanomaterials. By taking advantage of magnetic nanoparticles, nanobiocatalytic CA could be recycled for repetitive enzymatic conversion of CO₂ via facile magnetic separation. The present work has demonstrated a great potential of nanobiocatalytic CA as a stabilized enzyme system that can be employed for the biocatalytic CO₂ conversion and utilization.