474869 Chitosan Nanoparticles with Immobilized Glucose Oxidase As Efficient Antimicrobial Agents

Monday, November 14, 2016
Grand Ballroom B (Hilton San Francisco Union Square)
Jisung You1, Manab Deb Adhikari1, Seok-Joon Kwon2, Sung-Gil Hong1, Warayuth Sajomsang3, Jonathan S. Dordick2 and Jungbae Kim1, (1)Department of Chemical and Biological Engineering, Korea University, Seoul, Korea, The Republic of, (2)Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, (3)Nanoengineered Soft Materials for Green Environment Laboratory (SOF), National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathum Thani, Thailand

We have developed three immobilizations of glucose oxidase (GOx) on chitosan nanoparticles (CSNP), and investigated their antimicrobial activities against Staphylococcus aureus. CSNP were synthesized via ionotropic gelation, and GOx was immobilized on CSNP via three different approaches such as covalent attachment (CA), enzyme crosslinking (EC) and enzyme precipitation crosslinking (EPC). The activities of CA, EC and EPC were 8.72, 17.69, and 218.97 Unit/mg CSNP, respectively. According to the turbidity assay, we observed 90% growth inhibition in the presence of EPC when compared to the control with no enzyme added. Furthermore, EPC was the most effective in disintegrating the biofilm network of Staphylococcus aureus, resulting in more than 80% reduction in biofilm biomass. EPC on CSNP has shown a great potential in preventing microbial growth and disintegrating biofilms, which can find its applications in preventing microbial infections in medical implants and wound dressing materials.

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See more of this Session: Poster Session: Nanoscale Science and Engineering
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