473939 One Step Breaking Softwood Recalcitrance By Cellulose Solvent-Based Lignocellulose Fractionation Followed By Enzymatic Saccharification

Thursday, November 17, 2016: 1:20 PM
Mason (Hilton San Francisco Union Square)
Thanh Khoa Phung1, Li Kang2, Christian Canlas3, Scott Renneckar4, Teerawit Prasomsri5 and Noppadon Sathitsuksanoh1, (1)University of Louisville, Louisville, KY, (2)Auburn University, Auburn, AL, (3)King Abdullah University of Science and Technology, Thuwal, Saudi Arabia, (4)University of British Columbia, Vancouver, Canada, (5)MIT, Boston, MA

In our efforts to shift away from conventional petroleum-based feedstock to supply transportation fuels, plant biomass has emerged as an attractive renewable alternative. Softwoods have great potentials as renewable feedstocks for biorefineries. However, softwoods are more recalcitrant compared to hardwood counterparts. Hence, the use of softwoods in biorefineries has been viewed as limited. In the present study, we applied a cellulose solvent-based pretreatment/fractionation process on southern yellow pine and douglas fir at a mild condition: 50 ºC and 1 atm for 90 min. Pretreated softwood samples were hydrolyzed fast by cellulase (15 FPUs) and > 85% glucose yield was achieved by 24 h. Cellulose accessibility to cellulase of pretreated samples was probed by the fusion protein and was found to increase by 39 folds. After fractionation, degree of crystallinity of pretreated samples decreased and fibrous structure appeared to be disrupted as observed by solid-state 13C Cross-Polarization Magic Angle Spinning (CP/MAS) nuclear magnetic resonance (NMR) and scanning electron microscopy. No changes in lignin functionalities after fractionation were observed by 2D Heteronuclear Single-Quantum Correlation (HSQC) NMR, giving rise to the potential use of lignin as a co-product in biorefineries.

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See more of this Session: Recalcitrance of Woody Biomass
See more of this Group/Topical: 2016 International Congress on Energy