471982 Membrane-Based, Simple Filtration Device for Rapid Separation of Biological Particles from Complex Matrices
In this discussion, we demonstrate a simple viral or bacterial purification/concentration device that is easy to use, is reliable, and requires no energy for rapid viral separation. The device is comprised of two chambers, a feed chamber and a viral concentration chamber. Each chamber, which are 20 ml in volume, is divided with a poly (ethylene glycol) methacrylate (PEGMA) functionalized membrane to prevent membrane fouling and clogging. High water flux is maintained through driving forces from the packed polymer hydrogels in the concentration chamber and through gravitational pull. Filtration experiments with different sizes of colored-dye particles indicated that the device can exclusively separate small nanoparticles from large particles in mixture with a high filtration rate, up to 1.3 ml/min. In addition, the test results with dengue virus showed high filtration efficiency of the device with 90% of viral particle recovery and a concentration factor of 4.5. Our results suggest that the filtration device may provide a breakthrough toward the development of simple diagnostic platform for rapid detection of bloodstream infections, polymerase chain reaction sample preparation, and viral separation/concentration.