471718 Towards the Elucidation of the Limonin Biosynthetic Pathway, a Triterpene Derived Natural Product in Citrus sinensis
One important group of triterpene derivatives are the limonoids. Limonoids display potent insect-deterrent properties including anti-feedant, growth disruptive, and reproduction inhibitive activities. Limonoids exhibit high degrees of oxygenation and complex intermolecular rearrangements resulting from an estimate of a dozen unknown genes. Herein, we aim to elucidate the biosynthetic pathway of limonin, a prototypical limonoid found in Citrus sinensis (sweet or Valencia orange). We selected gene candidates via a homology and transcriptome analysis, and we developed a platform for rapid pathway discovery combining transient expression in the host Nicotiana benthamiana with liquid chromatography mass spectrometry (LC-MS) metabolomics. The utilization of this platform allows for fast screening of gene candidates potentially involved in the limonin biosynthetic pathway.