Catalase-Laden Microdevices for Cell-Mediated Enzyme Delivery

Catalase-Laden Microdevices for Cell-Mediated Enzyme Delivery

 

Junfei Xia¹, Zhibin Wang¹, Yuanwei Yan¹, Zhijian Cheng², Li Sun², Yan Li¹, Yi Ren², Jingjiao Guan^1,*

¹ Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University

² Department of Biomedical Sciences, College of Medicine, Florida State University

Abstract

Enzymes have been used as drugs to treat a wide variety of human diseases and traumas. However, therapeutic utility of free enzymes is generally impeded by short circulation time, lack of targeting ability, immunogenicity, and inability to cross certain biological barriers. Various strategies have been developed to overcome these limitations. Among them is cell-mediated drug delivery, which is featured by integrating drugs with live cells and taking advantage of the unique capabilities of the cells to achieve controlled drug delivery. We developed simple and inexpensive methods based on soft lithography and layer-by-layer (LbL) assembly for fabricating disk-shaped microparticles termed microdevices and generating the cell-microdevice complexes. [1, 2] We herein applied these methods to the fabrication of enzyme-laden microdevices and corresponding cell-microdevices complexes for the first time. We used catalase as a model enzyme in this study. It is a water soluble protein with an isoelectric point of 5.8.  It is thus negatively charged at pH 7. Poly(diallyldimethyl ammonium chloride) (PDAC) was chosen here as a polycation to form a bilayer with catalase. Catalase in the microdevices was catalytically active and active catalase was slowly released from the microdevices. The catalase-laden microdevices were attached to the external surface of live suspension and adhesion cells respectively to form cell-microdevice complexes. This technique is applicable to other therapeutic enzymes and therapeutic cells, and thus promises to find clinical applications for treating various human diseases and traumas.

 

References

1.    Junfei Xia, Zhibin Wang, Danting Huang, Yuanwei Yan, Yan Li, Jingjiao Guan*,Asymmetric Biodegradable Microdevices for Cell-Borne Drug Delivery, ACS Applied Materials & Interfaces (2015) 7: 6293-6299.

2.    Zhibin Wang, Junfei Xia, Yuanwei Yan, Ang-Chen Tsai, Yan Li, Teng Ma, Jingjiao Guan*,Facile functionalization and assembly of live cells with microcontact-printed polymeric biomaterials, Acta Biomaterialia (2015) 11: 80-87.