470835 High-Throughput, Low-Loss Dielectrophoretic Cell Separation

Monday, November 14, 2016: 10:45 AM
Embarcadero (Parc 55 San Francisco)
Shabnam Faraghat1, Max Steinbach1, Kai F. Hoettges1, Daan Van Der Veen1, Fatima H. Labeed2 and Michael P. Hughes2, (1)University of Surrey, Guildford, United Kingdom, (2)Mechanical Engineering Sciences, University of Surrey, Guildford, United Kingdom

Worldwide, the market for cell separation is dominated by three methods; fluorescence-activated cell sorting, magnetic-activated cell sorting, and density gradient methods; these variously suffer from drawbacks including high cell loss (typically >50%), the requirement for fluorescent or magnetic labels, and high cost (capital, operating or both). Dielectrophoresis was first used for cell separation over 50 years ago, and numerous systems for dielectrophoretically-activated cell sorting have been presented but few have matched existing methods for throughput and recovery. We have developed a cell enrichment system using the DEP-Well platform. Cells pass through 343 parallel wells containing 12 electrodes apiece, and energised by a 14Vpp signal at frequencies up to 1MHz. Processing speed is sufficiently high (in excess of 1 million cells per ml), and cell losses sufficiently low (below 5%), to enable multiple passes through the device in order to improve performance; on a second pass, separation efficiencies in excess of 95% are readily achievable; whilst on a third pass, recovery of rare cells becomes possible. Overall processing speed and recovery are comparable to or exceed FACS and MACS, whilst the use of a disposable chip avoids inter-separation contamination.

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