469672 Genome-Wide Genetic Screening in Mammalian Cells Using TALE Libraries

Monday, November 14, 2016: 2:00 PM
Continental 9 (Hilton San Francisco Union Square)
Yi Li1, Taek Kang2, Seth Lawson3 and Leonidas Bleris2, (1)Bioengineering, The University of Texas at Dallas, Richardson, TX, (2)Bioengineering, University of Texas at Dallas, Richardson, TX, (3)University of Texas at Dallas, richardson, TX

Genome-wide screens to identify genes connected to a phenotype of interest have been performed using RNAi libraries and recently CRISPR-Cas9 libraries. However, both approaches are limited by inherent disadvantages including off-target effects and inefficient gain-of-function screenings. Recently, we introduced an assembly method to efficiently construct TALE (transcription activator-like effectors) libraries which cover all possible combinations of the nucleotides, and demonstrated genome-wide screening experiments in yeast cells. In this study, we constructed biased TALE libraries to target desired motifs and subsequently performed genome-wide screens in human cells to probe for desired change in phenotype.

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See more of this Session: Genome Scale Engineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division