468430 Integrating Micro-Environmental Cues into Single-Cell Targeted Proteomics Tools
To accomplish this goal, we design, demonstrate, and characterize a microfluidic cell patterning technique that allows us to culture single cells and small cell clusters in controlled, tunable microenvironments and then directly analyze the lysate of each single cell in situ. Using short assay times, electrophoresis, and photo-activatable crosslinkers, we demonstrate separation, detection, and ratiometric analysis of protein targets from stem cells in diverse microenvironments. We describe our multi-layered (3D) microfluidic device, tunable polymer formulations (chemical, mechanical, geometric) and separations-related outcomes (dispersion, dilution, separation resolution, peak capacity). By integrating microenvironmental cues into a precision microanalytical instrument, we measure key morphometric and spatial information and correlate these physical data to protein expression patterns of each mammalian cell.
Looking forward, we view the combination of spatio-temporal information with targeted proteomic information as an important and, as of yet missing, axis of systems biology.
See more of this Group/Topical: 2016 Annual Meeting of the AES Electrophoresis Society