467852 ‘Omics Profiling of a Continuous Manufacturing CHO Production Platform for Monoclonal Antibodies
In this work, transcriptome and proteome profiling of a CHO cell line producing a mAb was performed to investigate the intracellular variability during steady-state of a perfusion reactor. Daily samples were analysed by high-throughput methods depicting the time evolution of several thousand transcripts and proteins. Data mining by different clustering methods allowed the analysis of each individual ‘omics study. Furthermore up- and down regulated intracellular processes and pathways responsible for the quality of the secreted mAb were investigated. By the use of bioinformatics databases the biological function was assigned and moreover key functions were characterized by functional annotation. Additionally, to identify differences between the mRNA and protein expression in time, an integrated data analysis of both datasets was completed, as the correlation of the proteomic and transcriptomic expression response is not necessarily given for every regulatory cell process. In conclusion, useful insights into cellular behaviour can be gained by the joint application of ‘omics technologies to address the intracellular process consistency of perfusion cell culture.