Trends in Preparative Chromatography

Besides the widespread application of chromatography as an analytical technique, there is a large and growing interest in the pharmaceutical industry and in biotechnology to isolate and purify value added products using preparative liquid chromatography. This led to remarkable progress in the areas of a) developing and commercializing highly selective and efficient stationary phases, b) understanding and quantifying the consequences of overloading chromatographic columns and c) exploiting more efficiently the driving forces for the separation using advanced operating regimes [1]. In the lecture selected new process concepts of preparative chromatography will be explained. Hereby, in particular multi-column configurations and various ways of exploiting gradients will be illustrated [2-5]. These concepts offer potential for significant improvements of essential performance parameters as productivity, yield and solvent consumption.

[1] Guiochon G., Felinger A., Shirazi D.G., Katti A.M., Fundamentals of Preparative and Nonlinear Chromatography, 2nd Edition, Academic Press, New York, 2006 [2] Seidel-Morgenstern A., Keßler L. C., Kaspereit M., New Developments in Simulated Moving Bed Chromatography, Chem. Eng. & Techn. 31, 2008, 826 [3] Seidel-Morgenstern A., Preparative Gradient Chromatography, Chem. Eng. & Techn. 28, 2005, 1265 [4] Schmidt-Traub H., Schulte M., Seidel-Morgenstern A. (Eds.), Preparative Chromatography, 2nd Edition, Wiley-VCH Verlag, Weinheim, 2012  [5] Kaspereit M., Seidel-Morgenstern A., Process Concepts in Preparative Chromatography, in: “Liquid Chromatography” (Eds. Fanali S., Haddad P., Poole C., Schoenmakers P., Lloyd D.), Elsevier, Oxford, 2013, Ch. 19, 427