467383 Crystallisation of Intact Monoclonal Antibody anticd20
Porous nanotemplates, tuned to the appropriate size, have been used to crystallise mAb anti cd20 by creating a spherulite seed stock. Templates have shown to significantly increase the number of crystals produced compared to crystallisation void of the heterogeneous nucleants. Stock solutions of these seeds were used for crystallisation and showed significant improvement in crystal quality. Moreover, seeding in different crystallisation conditions lead to various crystals with different morphologies.
- U.V. Shah, D.R. Williams, and J.Y.Y. Heng, “Selective Crystallization of Proteins Using Engineered Nanonucleants”,Cryst. Growth Des.,(2012), 12(3), 1362–1369.
- Lim, S.H., et al., Anti-CD20 monoclonal antibodies: historical and future perspectives. haematologica, 2010. 95(1): p. 135-143.