466897 Intensified Hydrolysis By Employing the Synergy of Mixed Subcritical H2o and Supercritical CO2

Monday, November 14, 2016: 2:30 PM
Franciscan A (Hilton San Francisco Union Square)
Armando Quitain1, Akito Hashimoto1, Saya Yamafuku1, Mitsuru Sasaki2 and Tetsuya Kida1, (1)Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan, (2)Institute of Pulsed Power Science, Kumamoto University, Kumamoto, Japan

Hydrolysis of naturally occuring compounds into more bioactive and bioavailable materials are gaining popularity in food and pharmaceutical industries. However, conventional method employs harmful catalysts such as sulfuric acid with low yield, while separation of the target compounds from the reaction products requires rather tedious approaches. To overcome these problems, this research aims to develop, optimize and evaluate by experimental approaches the combined reaction and separation process employing the synergy of subcritical H2O and supercritical CO2. Application to hydrolysis of citrus bioflavonoids (hesperidin) into its aglycones (hesperitin) was elucidated. Using a batch reactor, we were successful in hydrolyzing hesperidin into hesperetin under hydrothermal conditions with supercritical CO2. The highest yield of hesperetin was obtained at T=160 oC, P=15 MPa and t=4 h. Based on the results, a semi-batch reactor system was developed to study reaction and separation process employing the mixture of supercritical CO2 and subcritical H2O. After the reaction, extraction of hesperetin was carried out by passing supercritical CO2 through the reaction products. Results indicated the possibility of separating hesperetin from the mixture, and higher yield can be obtained by simply manipulating the pressure and temperature of CO2.

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