Synbiotics Effect of Probiotic Bacteria Isolated from Mother’s Milk and Different Types of Prebiotic for Short Chain Fatty Acids Production

Due to health promoting effects reported for probiotics, these strains are increasingly incorporated into functional food and pharmaceutical markets. The aim of this research is to study the growth and metabolism of probiotic strains isolated from mother’s milk in different types of prebiotic as a carbon source. The aim of this research to study the synbiotic effects between the probiotics bacteria and prebiotics to the production of SCFA such as acid, lactic acid, butyric acid, propionic acid and succinic acid. The probiotic bacteria isolated from mother milk used in this research were Lactobacillus salivarius and Lactobacillus delbreuckii bulgaricus in pure and mixed culture. The growth kinetics and metabolite profiling of SCFA were studied in different types of carbon sources such as inulin, FOS, gum Arabic and growth in glucose as a control. Between the four different types of carbon sources, the best growth profile was obtained by using inulin in mixed culture of L. bulgaricus and L. salivarius with maximum cell mass production of 8.23 g L^-1 and followed by FOS and glucose at the value of 7.67 g L^-1and 6.43 g L^-1 respectively, whereas specific growth rate for this culture was 0.33 h^-1. Furthermore, growth of L. salivarius in glucose gave the highest succinic acid production of 3.99 g L^-1 compared to other types of prebiotics and SCFA. However for cultivation of L. bulgaricus was produced the high amount of lactic acid that is 2.39g L^-1 after 24 h cultivation when glucose as the carbon source. Besides that, growth of mixed culture of L. bulgaricus and L. salivarius gave high succinic acid production almost 2.62 g L^-1in FOS after 24 h cultivation. Generally in this studied, it’s shown that growth of mixed culture of L. bulgaricus and L. salivarius in FOS also gave the highest amount of total SCFA that was 6.47g L^-1. As a conclusion, the correlation of the response would help to determine the choice of a good prebiotics for the probiotics strain in order to enhance the beneficial effect to human health.