464091 Engineering of Microbial Cell Factories for Biobased Chemicals Production

Monday, November 14, 2016
Grand Ballroom B (Hilton San Francisco Union Square)
Youyun Liang1, Shuobo Shi1, Hongfang Zhang1, Mingzi M. Zhang1, Zihe Liu1, Xiaoyun Su2, Tong Si3, Xiukai Li4, Yugen Zhang4, Huimin Zhao5 and Ee Lui Ang1, (1)Metabolic Engineering Research Laboratory, Agency for Science, Technology and Research, Singapore, Singapore, (2)Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China, (3)Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana Champaign, Urbana, IL, (4)Institute of Bioengineering and Nanotechnology, Agency for Science, Technology and Research, Singapore, Singapore, (5)Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL

The production of chemicals through biological systems offer promising solutions to global challenges presented by a carbon constrained future and an increasing global population. In the past decade, rapid advances in DNA sequencing and synthesis and genome editing technologies have accelerated our ability to program biological systems for the production bio-based chemicals from renewable feedstocks. However, challenges such as feedstock utilization, microbial cell factory engineering and stabilization of the production strain’s genetics, still remain.

The Metabolic Engineering Research Laboratory in Singapore is focused on addressing these challenges through the design and engineering of recombinant microorganisms. In this presentation, I will highlight our accomplishments including: (a) deconstruction of cellulose through engineering a Saccharomyces cerevisiae pentafunctional minicellulosome system with enhanced cellulose degradation capabilities, which can also be applied as a cellulase engineering platform, (b) metabolic engineering of microbial cell factories for the production of biochemicals, such as n-butanol and adipic acid, and (c) development of an efficient technological platform for the integration of large genetic pathways and circuits (up to 24 kb) into the yeast genomes to create genetically stable production hosts.

Extended Abstract: File Not Uploaded
See more of this Session: Poster Session: Bioengineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division