Plasma Immunoglobulins Drive Corona-Induced Negative Adhesion of Drug Carriers in Human Blood Flow

Plasma immunoglobulins drive corona-induced negative adhesion of drug carriers in human blood flow

Daniel J. Sobczynski and Omolola-Eniola-Adefeso The plasma protein corona has been identified to have deleterious effects on nanoparticle targeting efficiency to reactive substrates as well as human blood flow in vitro. Although protein size has been implicated in corona-induced negative targeting effects, the role of specific proteins in this process remains largely unknown. This work explores the role of immunoglobulin (Ig) proteins on prescribing the impact of the protein corona on inducing negative adhesion of PLGA drug carriers in blood flow via a parallel plate flow chamber assay. It is observed that binding of PLGA particles is restored upon depletion of immunoglobulins, in particular, IgG and IgA, however this is shown to depend on the human donor. Interestingly, no dependence of corona-derived effects on plasma donor Ig concentration is observed. Overall, this study reveals that the impact of the protein corona on PLGA drug carrier targeting efficiency is dominated by high abundant immunoglobulin proteins in blood plasma.