463508 Lipid Exchange Envelope Penetration (LEEP) of Nanoparticles for Plantengineering: A Universal Localization Mechanism

Thursday, November 17, 2016: 9:06 AM
Continental 6 (Hilton San Francisco Union Square)
Min Hao Wong1, Rahul Misra1, Juan Pablo Giraldo1,2, Seonyeong Kwak3, Youngwoo Son1, Markita Landry1,4, James Swan1, Daniel Blankschtein1 and Michael Strano5, (1)Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, (2)Department of Botany and Plant Sciences, University of California Riversides, Riversides, CA, (3)Department of Chemical Engineering, Massachusetts Institute of Technnology, Cambridge, MA, (4)Department of Chemical Engineering, University of California Berkeley, Berkeley, CA, (5)Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA

Many important plant functions, such as carbon dioxide reduction or energy generation is carried out within the chloroplast – a plant organelle that appears greatly under explored as an engineering material. Here, we examine the subcellular uptake and kinetic trapping of a wide range of nanoparticles for the first time, using the plant chloroplast as a model system, but validated in vivo in living plants. Confocal visible and near-infrared fluorescent microscopy and single particle tracking of gold-cysteine- AF405 (GNP-Cys- AF405), streptavidin-quantum dot (SA-QD), dextran and poly(acrylic acid) nanoceria, and various polymer-wrapped single-walled carbon nanotubes (SWCNTs), including lipid-PEG- SWCNT, chitosan-SWCNT and 30-base (dAdT) sequence of ssDNA (AT)15 wrapped SWCNTs (hereafter referred to as ss(AT)15-SWCNT), are used to demonstrate that particle size and the magnitude, but not the sign, of the zeta potential are key in determining whether a particle is spontaneously and kinetically trapped within the organelle, despite the negative zeta potential of the envelope. We develop a mathematical model of this lipid exchange envelope and penetration (LEEP) mechanism, which agrees well with observations of this size and zeta potential dependence. The theory predicts a critical particle size below which the mechanism fails at all zeta potentials, explaining why nanoparticles are critical for this process. LEEP constitutes a powerful particulate transport and localization mechanism for nanoparticles within the plant system.

Reference: Wong MH, Misra R, Giraldo JP, Kwak SY, Son YW, Landry MP, Swan JW, Blankschtein D, Strano MS. 2016. Lipid Exchange Envelope Penetration (LEEP) of Nanoparticles for Plant Engineering: a Universal Localization Mechanism. Nano lett, 16 (2), pp 1161–1172


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