462590 Modulation of Self-Assembly in Biological Motor Complexes

Tuesday, November 15, 2016: 4:45 PM
Market Street (Parc 55 San Francisco)
Ravi Chawla1, Katie Ford1 and Pushkar Lele2, (1)Texas A&M University, College Station, TX, (2)Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA

The flagellum in bacteria is a sensitive detector of perturbations in environmental viscosities. The driver for such sensing of mechanical signals is the flagellar motor, which tailors motility to suit the environment. Mechanosensitive torque-generating elements that rotate the motor detect mechanical signals, and reversibly remodel their assembly in response to changes in the viscous loads. Remodeling likely plays a role in triggering signaling pathways that control cell-morphology and motility. To determine the mechanism of remodeling and its role in signaling, we tested remodeling over varying viscous loads. We subjected wild-type strains and strains that lacked downstream-transducers of mechanical signals to large mechanical stimuli. From the initial speed-response (following the stimulus), we estimated the amount of torque-generated per stator-unit in the presence and absence of the effectors. Subsequent measurements revealed the absence of feedback from downstream effectors on motor remodeling. Although crucial for cell-survival and adaptation, proteins involved in downstream-signaling are unlikely to regulate motor-mechanosensing.

Extended Abstract: File Not Uploaded
See more of this Session: Bio-Fluid Dynamics
See more of this Group/Topical: Engineering Sciences and Fundamentals