462547 Investigating Biomembrane-Nanoparticle Interactions with Giant Vesicles Fabricated from Inverted-Headgroup Lipids
These interactions were investigated by fabricating giant unilamellar vesicles (GUVs) with DOPC lipids and DOCP lipids respectively, and introducing nanoparticles to suspensions of both. In the presence of charged polystyrene nanoparticles, GUVs displayed various deformational modes depending on the charge sign and size of the nanoparticles. The differences in the responses of the two lipid species were quantified according to the size changes of GUVs. These differences illuminate how the relative positions of the negative and positive charge groups on the lipid interact with charged particles. They also reveal a particle size dependence of the nanoparticle-membrane electrostatic interaction. This work provides fundamental insights regarding the nature of this interaction, which has implications for the development of new drug delivery vehicles as well as understanding the environmental toxicity of nanomaterials.