462269 Encapsulating Yeast in Agarose Microparticles for High Throughput Microcolony Analysis

Monday, November 14, 2016
Grand Ballroom B (Hilton San Francisco Union Square)
Leqian Liu, Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA and Adam R. Abate, Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA

Yeasts and fungi are powerful hosts to produce numerous value-added chemicals. Metabolic engineering efforts in yeasts can be coupled with high-throughput analysis platform to effectively analyze and select desired phenotypes. However, choices of analyzing yeast cells using high-throughput are still limited. Traditional plate based colony screening can be non-quantitative and time-consuming while FACS based single cell screening can be associated with high false positive rate. To provide an alternative method for high-throughput analysis in yeasts, we performed experiments in a droplet microfluidic platform. We encapsulated single yeast cells into pico to nano-liter sized agarose droplets and allowed them to culture and to form isolated microcolonies in agarose microparticles. These microparticles can then be analyzed by high-throughput analysis platform such as flow cytometry and next generation sequencing. This workflow enables a quantitative and high-throughput analysis of yeast colonies.

Extended Abstract: File Not Uploaded
See more of this Session: Poster Session: Bioengineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division