458544 Repurposing Endogenous Crispr-Cas Bacterial Immune Systems for Programmable Gene Repression
Utilizing this platform, we explored how the length of the CRISPR RNA impacts the form and function of the Type I-E effector protein complex called Cascade. We discovered that the length of the Type I-E CRISPR RNA molecule is not fixed and can be extended, altering the stoichiometry of the Cascade effector complex while preserving functionality. Interestingly, altering RNA length can elicit significant improvement in transcriptional silencing efficiency, but only for particular target locations. These findings heighten our understanding of CRISPR-Cas systems as they continue to increase in popularity and may offer insight into designing tunable transcriptional regulators.