452148 Separation of Carotenoids By ULTRA High Performance Supercritical FLUID Chromatography (UHPSFC)

Wednesday, November 16, 2016: 3:40 PM
Cyril Magnin II (Parc 55 San Francisco)
Cesar Costapinto Santana1, Luciana Guedes2, Isabel Jardim3 and Marcia Breitkreitz3, (1)Biotechnological Processes, State University of Campinas, Campinas, Brazil, (2)Chemical Engineering, (3)Analytical Chemistry

Design of experiments and experimental data obtained in analytical equipment were applied to study separation of carotenoids mixtures by ultra high performance supercritical fluid chromatography (UHPSFC). A statistical strategy using response surface methodology for the separation of lycopene, beta-carotene, coenzyme Q10 and lutein was developed for the supercritical fluid chromatography technique. The design of experiments was employed to investigate the individual and interaction effects of temperature, pressure, and ethanol co-solvent percentage with respect to retention and selectivity. Central composite design was used to identify and optimize the significant factors. In the designed experiments, the temperature was varied within the interval 25-50 oC. The pressure and was set between 1500 and 2200 psi and the ethanol percentage between 15 and 24 (v/v) %. Each variable was tested at three levels and 19 experiments were performed in random order. It was found for the investigated model system that the ethanol percentage and pressure were the parameters that mostly affected the retention factor while the three parameters affected the separation factor. Considering both the the retention and separation factors, the selected operational conditions for further experiments were 15.5% of ethanol at 40 oC and 1500 psi, which has been shown to be inside the robust operating region.

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