441762 Production and Characterization of Hydrophobins from Fungal Source

Monday, November 9, 2015
Exhibit Hall 1 (Salt Palace Convention Center)
Chaitanya Habib, Sona Umloti, Vaibhav Hiregoudar and Saiprasad Wandkar, Biotechnology, BVBCET, Hubli, India

Hydrophobins are a large family of small cysteine rich proteins with molecular mass less than 20 kDa produced by filamentous fungi. These hydrophobins are reported to help in the formation of aerial hyphae and various other vital functions. The name hydrophobins was originally used due to their high content of hydrophobic amino acids. One of its important properties is the self assembly; the term molecular self-assembly refers to the autonomous formation of structures or patterns from pre-existing components. Due to these self assembly and amphipathic properties, hydrophobins find wide applications in food industry, breweries, pharmaceutical industries, etc. The present work reports on the production of hydrophobins from fungal source and their initial characterization. Production of hydrophobins from selected fungal strains (Trichoderma ressei, Trichoderma viridae, Trichoderma harzanium and white rot fungus) was undertaken at flask level. Extraction of hydrophobins was done by cell lysis by ultrasonic treatment. Extracted Hydrophobin in the foam were qualitatively analyzed for protein content. Hydrophobin is a polypeptide containing eight cysteine residues and presence of cysteine in hydrophobins was confirmed by standard lead sulphide test. The protein content was analyzed by standard Bradford method. Primary purification was done by ultrafiltration and characterization studies were done using SDS PAGE. The quantification studies revealed that Trichoderma ressei produced highest concentration of Hydrophobin (0.25mg/100ml) after 72 hours of incubation. Study of effect of carbon sources on production of hydrophobins by Tricoderma ressei was carried out which showed glucose to be the preferred carbon source. As there are only few reports, present work is significant towards laboratory level production of fungal hydrophobins.

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