439319 Electrokinetically Driven Microfluidic Analyzers

Wednesday, November 11, 2015: 4:45 PM
Ballroom E (Salt Palace Convention Center)
Adam T. Woolley, Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT

We are making micromachined systems that combine several analysis processes, with the objective of measuring disease-linked biomolecules. We photopolymerize porous polymer monoliths within microfluidic channels for solid-phase extraction involving either immunoaffinity or reversed-phase interactions. Then, purified, concentrated target compounds are analyzed through microchip electrophoresis. This approach was initially demonstrated with electrically driven extraction and electrophoretic analysis of multiple proteins related to cancer [1]. We have also implemented reversed-phase monoliths for electrokinetic on-chip extraction and fluorescence labeling of proteins [2]. Recently, we developed electrophoresis microdevices that have integrated pneumatic valves and pumps for automated pressure-based injection of precise solution volumes. We have also demonstrated field-amplified stacking and injection of non-aqueous samples in these microchips. Our present focus entails the application of these integrated microfluidic systems in studying a panel of preterm birth biomarkers. These microdevices should be useful in the analysis of small-volume biological samples. 

[1] Yang, W.; Yu, M.; Sun, X.; Woolley, A.T. Lab Chip 10, 2527–2533 (2010).
[2] Yang, R.; Pagaduan, J.V.; Yu, M.; Woolley, A.T. Anal. Bioanal. Chem. 407, 737-747 (2015).

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