435583 Dynamic Self-Assembly of Motile Bacteria in Liquid Crystals

Tuesday, November 10, 2015: 9:30 AM
Canyon B (Hilton Salt Lake City Center)
Nicholas L. Abbott, Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, Peter Mushenheim, Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Douglas B. Weibel, University of Wisconsin-Madison, Madison, WI and Rishi Trivedi, Department of Biochemistry, University of Wisconsin-Madison, Madison

This presentation will describe an investigation of dynamical behaviors of motile rod-shaped bacteria within anisotropic viscoelastic environments defined by lyotropic liquid crystals (LCs).  In contrast to passive microparticles (including non-motile bacteria) that associate irreversibly in LCs via elasticity-mediated forces, we report that motile Proteus mirabilis bacteria form dynamic and reversible multi-cellular assemblies when dispersed in a lyotropic LC.  By measuring the velocity of the bacteria through the LC (8.8 +/- 0.2 micrometer/s) and by characterizing the ordering of the LC about the rod-shaped bacteria (tangential anchoring), we conclude that the reversibility of the inter-bacterial interaction emerges from the interplay of forces generated by the flagella of the bacteria and the elasticity of the LC, both of which are comparable in magnitude (tens of pN) for motile Proteus mirabilis cells.  We also measured the dissociation process, which occurs in a direction determined by the LC, to bias the size distribution of multi-cellular bacterial complexes in a population of motile Proteus mirabilisrelative to a population of non-motile cells.  Overall, these observations and others reported in this paper provide insight into the fundamental dynamical behaviors of bacteria in complex anisotropic environments and suggest that motile bacteria in LCs are an exciting model system for exploration of principles for the design of active materials.

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See more of this Session: Active Colloidal Systems I
See more of this Group/Topical: Engineering Sciences and Fundamentals