435319 Surface and Rheological Effects of Mucus/Mucin Coupled with Chitosan-Coated Gold Nanoparticles

Monday, November 9, 2015
Exhibit Hall 1 (Salt Palace Convention Center)
Erick S. Vasquez1,2, Elizabeth Duggan3, Jordan Metcalf3, Santanu Kundu1 and Keisha B. Walters1, (1)Dave C. Swalm School of Chemical Engineering, Mississippi State University, Mississippi State, MS, (2)Department of Chemical and Materials Engineering, University of Dayton, Dayton, OH, (3)Pulm. & Crit. Care Div./Dept. Med., Oklahoma University Health Sciences Center, Oklahoma City, OK

We have examined surface-functionalized gold nanoparticles (Au NPs) within mucin/mucus matrices to gain a better understanding of the mechanical and chemical interactions, as these systems are model nanoparticle-based therapeutics.  Specifically, glycol-chitosan (GC) surface moieties were added to the Au NPs using a one-pot synthesis method where glycol-chitosan acts as both a stabilizer and a reducing agent. Particle size distributions, stability, and structure of the ‘as synthesized’ Au NPs and glycol-chitosan modified Au NPs (GC-Au) were measured using dynamic light scattering (DLS) and ultraviolet visible spectroscopy (UV-Vis). After confirming the successful synthesis of GC-Au nanoparticles, mucin aliquots were added to the GC-Au nanoparticle solution.  Changes in the particle size were monitored using DLS to examine complex formation and binding interactions between the GC-Au nanoparticles and mucin. By varying pH, complexation time, concentration, and temperature, the stability of the mucin-chitosan Au nanoparticle complexes was evaluated.  Morphological characteristics of these hybrid systems were examined using TEM, and net-surface charge effects of the nanoparticle interacting with mucin was characterized by z-potential measurements. In addition, we have examined the bulk rheological effects for synthetic mucus solutions—based on pig gastric mucin (PGM) and different stabilizers—in response to GC-Au nanoparticle addition at different concentrations. This work provides foundational information on interactions between surface-functionalized nanoparticles and biopolymers, which is important in developing next-generation biomedical therapeutics and theranostics.

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