431925 Effect of C/N Ratio on Enzymatic Activity for Microbial Lipid Production

Tuesday, November 10, 2015: 12:30 PM
257B (Salt Palace Convention Center)
Ramalingam Subramaniam1, Markus Esser2, Milan Popovic2, Johannes Bader2, Andrei Chistoserdov3 and Rakesh Bajpai1, (1)Department of Chemical Engineering, University of Louisiana at Lafayette, Lafayette, LA, (2)Beuth University of Applied Sciences, Berlin, Germany, (3)Department of Biology, University of Louisiana at Lafayette, Lafayette, LA

The main factor affecting lipid accumulation by microorganisms is a high C/N-ratio and the concentration of the trace elements K+, Ca2+, Zn2+, Fe3+ and Mn2+. While deficiencies in such nutrients as nitrogen, sulfur, phosphorus, and iron have been reported as being able to cause cell growth cessation and channeling of metabolic flux to lipid/fatty acid biosynthesis, the most commonly used limiting-nutrient is nitrogen as it is easily controllable through media composition. Although this fact has been well documented, most of the reports deal with the effect of initial C/N-ratio on the ultimate lipid accumulation by cells in batch cultures. This work deals with the effect of C/N-ratio and specific growth rate of cells on the activities of key enzymes of lipid pathway and on lipid accumulation under steady state conditions.

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