431134 Performing Gene Mapping on DNA Unraveled on Patterned Lipid Bilayers

Tuesday, November 10, 2015: 2:00 PM
Ballroom E (Salt Palace Convention Center)
Hou-Jun Guo, Hung-En Lee and Chih-Chen Hsieh, Chemical Engineering Department, National Taiwan University, Taipei, Taiwan

We have developed a new platform for stretching DNA and performed rapid gene mapping on the platform using various labeling methods. In this study, DNA were first adsorbed on cationic-lipid membranes that are supported on grooved glass surface. The adsorbed DNA then spontaneously gathered and unraveled along the grooves. For both T4GT7(166kbp) and lambda DNA(48.5kbp), their relative extension can reach as high as 85%, comparable to the value obtained as DNA were confined in sub-50nm nanochannels. On this platform, we have performed gene mapping on both lambda and T4GT7 DNA using several labeling approaches including restriction digestion, fluorescent peptide nucleic acids, and nick translation. The locations of labeling agents (or the corresponding sequences) on DNA were determined using fluorescence microscopy. All three labeling methods were found compatible with the lipid platform and their results are at similar degree of accuracy. Since our method is faster and cheaper than the traditional ones, it has great potential for future gene mapping applications.

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