430669 Self-Folding Single Cell Grippers

Monday, November 9, 2015: 9:42 AM
151A/B (Salt Palace Convention Center)
Beril Polat, Kate Malachowski, Qianru Jin and David H. Gracias, Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD

SELF-FOLDING SINGLE CELL GRIPPERS

Beril Polat

(As the 4th author)

bpolat3@jhu.edu

The Johns Hopkins University

Department of Chemical and Biomolecular Engineering

ABSTRACT:

Given the heterogeneous nature of cultures, tumors, and tissues, the ability to capture, contain, and analyze single cells is important for genomics, proteomics, diagnostics, therapeutics, and surgery. Moreover, for surgical applications in small conduits in the body such as in the cardiovascular system, there is a need for tiny tools that approach the size of the single red blood cells that traverse the blood vessels and capillaries. We describe the fabrication of arrayed or untethered single cell grippers composed of biocompatible and bioresorbable silicon monoxide and silicon dioxide. The energy required to actuate these grippers is derived from the release of residual stress in 3-27 nm thick films, did not require any wires, tethers, or batteries, and resulted in folding angles over 100 with folding radii as small as 765 nm. We developed and applied a finite element model to predict these folding angles. Finally, we demonstrated the capture of live mouse fibroblast cells in an array of grippers and individual red blood cells in untethered grippers, which could be released from the substrate to illustrate the potential utility for in vivo operations.


Extended Abstract: File Uploaded
See more of this Session: Tissue Engineering Microenvironment
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division