429386 Tuning Nanoparticle Elasticity for Improved Biological Function

Thursday, November 12, 2015: 9:24 AM
251A (Salt Palace Convention Center)
Aaron C. Anselmo1, Mengwen Zhang2, Sunny Kumar1, Douglas R. Vogus2, Stefano Menegatti3, Matthew E. Helgeson2 and Samir Mitragotri1, (1)Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, CA, (2)Chemical Engineering, University of California, Santa Barbara, Santa Barbara, CA, (3)Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC

The physical and chemical attributes of nanoparticles have been systematically investigated to determine their role in key drug delivery processes such as circulation, targeting, and cellular uptake. These efforts have shown that physical modifications (e.g. tuning size or shape) can be used to influence the biological fate and function of nanoparticles and improve the drug delivery process. However, the role nanoparticle flexibility has on their biological function and fate has been less explored. As such, the potential benefits of tuning nanoparticle elasticity are not clear and similar systematic investigations are warranted. By synthesizing polyethylene glycol (PEG)-based hydrogel nanoparticles of uniform size (200 nm) with elastic moduli ranging from 0.255 kPa to 3000 kPa, we have investigated the role of particle elasticity on key functions including circulation time, tissue targeting, and cellular internalization. We show that softer nanoparticles (10 kPa) offer improved circulation and enhanced targeting compared to stiffer nanoparticles (3000 kPa) in vivo. In vitro studies showed that stiffer nanoparticles were internalized in significantly higher amounts than their softer counterparts in a variety of cell types. Tuning nanoparticle elasticity offers a new method to improve the circulation and targeting of nanoparticle drug delivery systems.

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See more of this Session: Biomaterials for Drug Delivery
See more of this Group/Topical: Materials Engineering and Sciences Division