427197 Membrane Surface-Enhanced Raman Spectroscopy for High-Sensitive Detection of Molecular Behavior of Lipid Assemblies

Wednesday, November 11, 2015: 2:15 PM
Canyon B (Hilton Salt Lake City Center)
Keishi Suga1, Tomohiro Yoshida1, Haruyuki Ishii2, Yukihiro Okamoto1 and Hiroshi Umakoshi1, (1)Div. of Chem. Eng., Grad. Sch. of Eng. Sci., Osaka University, Toyonaka, Japan, (2)Dep. of Chem. Eng., Grad. Sch. of Eng., Tohoku University, Sendai, Japan

The dynamic properties of phospholipid (PL) membranes (phase state and phase transition) play crucial roles in biological systems. However, highly sensitive, direct analytical methods that shed light on the nature of lipids and their assemblies have not been developed to date. Here, we describe the analysis of PL-modified Au nanoparticles (Au@PL) using membrane surface-enhanced Raman spectroscopy (MSERS) and report the properties of the self-assembled PL membranes on the Au nanoparticle. The Raman intensity per PL concentration increased by 50−170 times with Au@PL, as compared to large unilamellar vesicles (LUVs) at the same PL concentration. The phase state and phase transition temperature of the PL membrane of Au@PL were investigated by analyzing the Raman peak ratio (R = I2882/I2930). The enhancement at 714 cm−1 (EF(714)) varied with the hydrocarbon chain length of the PLs and the assembled degree of Au@PLs. In calculation, the EF(714),assembled was estimated to be 111−142 when the distance between AuNPs was 7.0−7.5 nm, which was correlated to the speculative enhancement factor, suggesting that the assembly of the Au@PLs contributed to the MSERS.

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See more of this Session: Self-Assembly in Solution
See more of this Group/Topical: Engineering Sciences and Fundamentals