Wednesday, November 11, 2015: 12:30 PM
155C (Salt Palace Convention Center)
Rhamnolipid biosurfactants are natural surfactants, emulsifiers, and a promising alternative to synthetic surfactants. A newly isolated Pseudomonas aeruginosa BC1 from oil-field wastewater was able to produce a glycolipid biosurfactant (i.e., rhamnolipid). Thin layer chromatotography (TLC), fourier transform infrared spectroscopy (FT-IR) and high performance liquid chromatography-mass spectrometry (HPLC-MS) worked together to reveal that the biosurfactant consisted mainly of mono-rhamnolipid (RL1). The present work attempted to enhance RL1 production by Pseudomonas aeruginosa BC1 in a pervaporation membrane bioreactor with the convenient permeate substances separation. The results showed that the enhanced fermentation could be achieved with high production of RL1 and variety of volatile substances separation in situ. Without volatile substances separation, RL1 production was 0.942g/l and 1.54g/l when glucose and glycerol as carbon source, respectively. However, in pervaporation membrane bioreactor, RL1 production reached 1.3g/l and 3.6g/l, an improvement of 38% and 134%, respectively. During the fermentation process in pervaporation membrane bioreactor, the convenient permeate substances separation in situ improved the ability of cell growth and metabolism obviously. Oil displacement experiments showed 76% heavy oil stripping from artificial quartz sands.