416971 Hierarchically Patterned Microfibers Via 3D Jet Writing

Wednesday, November 11, 2015: 1:24 PM
251A (Salt Palace Convention Center)
Jacob Jordahl1, Luis Solorio1, Hongli Sun2, Stacy Ramcharan1, Clark Teeple3, Paul Krebsbach4 and Joerg Lahann5, (1)Chemical Engineering, University of Michigan, Ann Arbor, MI, (2)University of South Dakota, Vermillion, SD, (3)Mechanical Engineering, University of Michigan, Ann Arbor, MI, (4)University of Michigan, Ann Arbor, MI, (5)Department of Chemical Engineering, Macromolecular Science and Engineering, Biomedical Engineering, and Materials Science and Engineering, University of Michigan, Ann Arbor, MI

Electrospinning of polymer fibers is a technique that has been in use for nearly a century, and has recently been a popular platform for creating tissue engineering scaffolds. However, the use of these fibers is quite limited due to the inability to precisely control the fiber architecture. Electrohydrodynamic (EHD) co-jetting is a method of creating compartmentalized polymer fibers which allows multiple surface chemistries, mechanical properties, polymer degradation, or drug loadings to be confined into separate continuous compartments in a single fiber. Utilization of EHD co-jetting combined with a newly developed method for patterning polymer fibers via an ultra-stabilized jet has been used to create hyper-porous polymer scaffolds of multi-compartmental fibers. The combination of multicompartmental fibers and the direct fiber writing process provides a platform for controlled anisotropy, geometries, pore sizes, surface functionalities, and mechanical gradients independently on a single 3D structure. These scaffolds provide a platform technology that can be used to modulate multiple parameters simultaneously and repeatedly to more accurately recapitulate a cell's native environment. To demonstrate the fiber scaffolds can produce viable engineered tissues, the scaffolds were used to culture human mesenchymal stem cells (hMSCs) into thick tissue-like sheets. The hMSCs were subsequently osteogenically differentiated, and were implanted into a calvarial defect in nude mice for eight weeks. This system was able to generate new bone across a 3 mm defect in the mouse's skull using differentiated hMSCs grown on the fiber scaffolds.

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See more of this Session: Biomaterial Scaffolds for Tissue Engineering
See more of this Group/Topical: Materials Engineering and Sciences Division