398572 Construction of Gene Deletion Mutants in Teredinibacter Turnerae to Determine Gene Function

Monday, November 17, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Jessica Steigerwald1,2, Hiroaki Naka2 and Margo Haygood2, (1)Oregon State University, Corvallis, OR, (2)Institute of Environmental Health, Oregon Health and Science University, Portland, OR


Teredinibacter turnerae is a bacterial endosymbiont of marine bivalve mollusks of the family Teredinidae1T. turnerae has the ability to fix nitrogen and to use cellulose as a carbon source, an asset which it confers to its host as well1. This bacterium also produces anti-microbial compounds to inhibit the growth of transient microbes in shipworms2. Sequencing of the T. turnerae (strain T7901) genome was recently completed, and has led to the identification of nine polyketide synthesis or non-ribosomal peptide synthesis biosynthesis regions potentially encoding bioactive compounds1.  So far, only two regions are characterized.  Region two is known to code for production of tartralon (an anti-microbial compound), while region seven is known to code for turnerbactin (a triscatecholate siderophore) 1.  In this study, I construct two separate single gene deletion mutants (ΔTERTU_1990 and DTERTU_2288,located in regions one and three respectively) to aid in the characterization of the genes.


1. Yang, J. C. et al. The complete genome of Teredinibacter turnerae T7901: an intracellular endosymbiont of marine wood-boring bivalves (shipworms). PloS One 4,e6085 (2009).

2. Trindade-Silva, A. E. et al. Physiological traits of the symbiotic bacterium Teredinibacter turnerae isolated from the mangrove shipworm Neoteredo reynei. Genet. Mol. Biol. 32, 572–581 (2009).

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