395904 The Effects of 4-Hydroxynonenal on Reactive Oxygen Species Generation By Pressurized Macrophages

Monday, November 17, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Marcos N. Barcellona, Bioengineering, University of Missouri - Columbia, Columbia, MO, Thomas D. Dziubla, Chemical and Materials Engineering, University of Kentucky, Lexington, KY and Hainsworth Y. Shin, Center for Biomedical Engineering, University of Kentucky, Lexington, KY

Previous studies demonstrated that exposure of macrophages to pressure fluctuations alters their capacity to generate reactive oxygen species (ROS).  For example, it has been determined that in vitro exposure of macrophages to 20 mmHg above ambient pressure can lead to a decrease in ROS generation as well as parallel changes in F-actin distribution.  Different chemicals exhibiting concentration dependent pro-oxidant characteristics, such as quercetin and trolox, have previously been explored as a means to counteract the anti-oxidant effects of pressure on ROS generation.  Interestingly, these chemicals at concentrations previously shown to stimulate ROS generation failed to recover ROS in pressurized macrophages.  In the present study, we used 4-Hydroxynonenal (4-HNE) as an alternative approach, as it has previously been demonstrated to stimulate ROS generation in macrophages.  Macrophages were exposed to 20 mmHg above ambient pressures for 2 hours and assessed for their ROS production and F-actin organization.  We determined that although 4-HNE may be used in order to recover basal ROS levels in pressurized cells, it was incapable of counteracting the suppressive effects of pressure.  We also observed that pressure-sensitive reorganization of F-actin was unaffected by 4-HNE.  Overall, our results suggest that the effects of pressure on macrophage ROS generation and F-actin distribution are independent of 4-HNE.

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