390133 The Ion Exchange Proteome of Pseudomonas Fluorescens

Wednesday, November 19, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Ahmed El Mashiete, Cell and Molecular Biology / Chemical Engineering, University of Arkansas, Fayetteville, AR and Bob Beitle, Ralph E. Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR

Pseudomonas fluorescens is gaining popularity as an expression host for a variety of recombinant DNA products that range in application from therapeutics to industrial enzymes.   In this poster, we present a characterization of the sub-proteome of P. fluorescens that interact with ion exchange chromatography.  Batch and fed-batch cultures of P. fluorescens that overexpress Green Fluorescent Protein, a model product, provided extracts that were fractionated by DEAE chromatography.  The proteins encountered during chromatography were identified, and a strategy was developed to improve GFP recovery (purity and column capacity).


Extended Abstract: File Not Uploaded
See more of this Session: Poster Session: Pharmaceutical
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division