389867 Reducing Host Cell Burden By Strategic Genome Design

Monday, November 17, 2014: 9:24 AM
205 (Hilton Atlanta)
Bob Beitle1, Ellen M. Brune2, McKinzie Fruchtl2, Mohammad M. Ataai3 and Ralph Henry4, (1)Ralph E. Martin Department of Chemical Engineering, University of Arkansas, Fayetteville, AR, (2)Boston Mountain Biotech, Fayetteville, AR, (3)Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, (4)Department of Biological Sciences, University of Arkansas, Fayetteville, AR

The design of Escherichia coli to improve bioprocessing is the subject of this presentation, a project which combines biochemical engineering, bioseparation, and proteomics.  Since downstream processing typically comprises a substantial portion of development and manufacturing, improvements in cell lines that reduce host cell burden is greatly desired.  Our efforts to improve ion exchange chromatography and immobilized metal affinity chromatography has demonstrated the fact that by understanding and manipulating the proteome of the host, improvements in purity and more importantly, column capacity can be realized.  During this presentation, a mathematical framework will be described that is used to guide host cell changes.  The design, construction, and deployment of this framework will be shown to be effective by various illustrative case studies of E. coli expression and purification.  Finally, we will demonstrate significant increases in column capacity – ion exchange in particular – and discuss the value of the approach to academia and industry.

Extended Abstract: File Not Uploaded
See more of this Session: Bioseparations and Downstream Processing
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division