389462 Investigating the Physiological Implications of a Nanotopographically-Induced Alignment of Endothelial Cells

Wednesday, November 19, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Jennifer Fischer1, Allison Murner1, Lindsay Gray1, Thomas Dziubla1, Christine Trinkle2 and Kimberly W. Anderson1, (1)Chemical and Materials Engineering, University of Kentucky, Lexington, KY, (2)Mechanical Engineering, University of Kentucky, Lexington, KY

It has recently been suggested that the nanotopography of a surface might alone be sufficient to promote a more physiologically-relevant morphology and surface chemistry of endothelial cells in vitro, thereby eliminating the need for cumbersome flow adaption efforts. In this work, we investigated the efficacy of a groove-aligned human umbilical vein endothelial cell (HUVEC) monolayer to determine if nanotopography alone could create a better mimic of the in vivo endothelium than statically cultured cells on standard glass substrates. Results suggest that while nano-scale grooves can be implemented to ensure a more unidirectional, elongated growth of HUVECs, the surface expressions of four common leukocyte and tumor cell adhesion molecules (ICAM-1, VCAM-1, E-selectin, P-selectin) were not significantly upregulated as compared to traditional cytokine-induced upregulation by tumor necrosis factor alpha (TNF-α).  The inherent decoupling of morphology and surface chemistry highlights an important structure/function mismatch incurred when generating biomimetic devices that should not be overlooked.

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See more of this Session: Poster Session: Bioengineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division