389353 Nanotemplated Polyelectrolyte Films As Porous Biomolecular Delivery Systems

Friday, November 21, 2014: 8:30 AM
International 3 (Marriott Marquis Atlanta)
Adeline Gand1, Mathilde Hindie1, Diane Chacon1, Emmanuel Pauthe1 and Paul R. Van Tassel2, (1)Université de Cergy-Pontoise, Cergy-Pontoise, France, (2)Yale University, New Haven, CT

Biomaterials capable of delivering controlled quantities of bioactive agents, while maintaining mechanical integrity, are needed for a variety of cell contacting applications. We describe here a nanotemplating strategy toward porous, polyelectrolyte-based thin films capable of controlled biomolecular loading and release. Films are formed via the layer-by-layer assembly of charged polymers and nanoparticles (NP), then chemically cross-linked to increase mechanical rigidity and stability, and finally exposed to tetrahydrofuran to dissolve the NP and create an intra-film porous network. We report here on the loading and release of the growth factor bone morphogenetic protein 2 (BMP-2), and the influence of BMP-2 loaded films on contacting murine C2C12 myoblasts. We observe nanotemplating to enable stable BMP-2 loading throughout the thickness of the film, and find the nanotemplated film to exhibit comparable cell adhesion, and enhanced cell differentiation, compared to a non-porous cross-linked film (where BMP-2 loading is mainly confined to the film surface).

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See more of this Session: Biomolecules at Biomaterial Interfaces
See more of this Group/Topical: Materials Engineering and Sciences Division