388510 Enhancing Fatty Acid Ethyl Ester Production in Saccharomyces Cerevisiae through Metabolic Engineering and Culture Optimization

Thursday, November 20, 2014: 2:06 PM
214 (Hilton Atlanta)
R. Adam Thompson, Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, Knoxville, TN and Cong T. Trinh, Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, Knoxville, TN

Biodiesels in the form of fatty acyl ethyl esters (FAEEs) are a promising next generation biofuel due to their chemical properties and compatibility with existing infrastructure. It has recently been shown that expression of a bacterial acyl-transferase in the established industrial workhorse Saccharomyces cerevisiae can lead to production of FAEEs by condensation of fatty acyl-CoAs and ethanol. We report a series of novel metabolic engineering strategies for FAEE production in S. cerevisiae which were rationally designed to pull the acyl-CoA substrate from competing pathways towards product formation. In addition, we combined our engineered strain with a modification of culture conditions designed to increase lipid accumulation in yeast. These strategies acted together to increase FAEE production over 17 fold.

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