388310 Targeted Binding of Nanolipoprotein Particles to Phase Separated Lipid Domains

Thursday, November 20, 2014: 12:50 PM
International 5 (Marriott Marquis Atlanta)
Wade F. Zeno and Marjorie L. Longo, Chemical Engineering and Materials Science, University of California, Davis, Davis, CA

Targeted binding of functional proteins and/or biomolecules to supported lipid bilayers has a variety of applications, including development of nano-array technologies and biosensors. Use of Integral Membrane Proteins (IMPs) in such systems is difficult, as incorporation into planar bilayers is not straightforward and can result in protein inactivation. We intend to overcome this limitation by use of Nanolipoprotein Particles (NLPs), which are able to solubilize IMPs. NLPs are discoidal patches of lipid bilayer that have their hydrophobic faces covered by amphiphilic scaffold proteins. The scaffold proteins can bear polyhistadine tags, which are capable of chelating to Cu2+ metal ions. Phase specific, Iminodiacetic acid functionalized lipids are also capable of chelating Cu2+, providing a mechanism for targeted binding of NLPs. We investigate this binding via fluorescence microscopy and characterize interaction on supported bilayers and giant unilamellar vesicles (GUVs). The thermodynamics (enthalpy of lipid mixing and steric pressure of protein crowding) and morphology of binding are also examined.

Extended Abstract: File Not Uploaded
See more of this Session: Self-Assembled Biomaterials
See more of this Group/Topical: Nanoscale Science and Engineering Forum