387244 Novel Antifungal Fusion Proteins
Development of recombinant platform to express novel antifungal peptides with the use of an anchor protein forms the motivation for the present work. Although numerous soluble peptide signaling molecules are known, there are very few options of psuedobiospecific anchor peptides useful for the development of new fusion proteins. OmpL37, a protein found on the surface of leptospire spp. is hypothesized to be useful for the binding of biologics to skin, as it has strong preferential affinity for components of the (epi)dermis while giving a nil immune response.
This poster will discuss the methodology of developing OmpL37-based constructs for the effective delivery of a novel antifungal peptide. The poster describes the cloning strategy of intermediate (GFP-based affinity) and final sequences, defines conditions for fed-batch cultivation (media and inducing strategies), and examines efficacy of the antifungal.