387028 Metabolic Engineering of Saccharomyces Cerevisiae for Enhanced Carotenoid Production

Wednesday, November 19, 2014
Galleria Exhibit Hall (Hilton Atlanta)
Michelle Olson, Luis H. Reyes and Katy Kao, Department of Chemical Engineering, Texas A&M University, College Station, TX

Metabolic engineering was utilized in an aim to optimize and study the production of carotenoids, specifically β-carotene, in engineered Saccharomyces cerevisiae strains. Our group has previously developed a novel evolutionary engineering approach to obtain hyper producer strains of isoprenoid compounds. Carotenoid production in yeast uses the cystolic mevalonate (MVA) pathway. In the MVA pathway, the catalytic domain of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) converts HMG-CoA to mevalonate.  This HMG-CoA reductase is a rate-limiting step in isoprenoid biosynthesis and overexpression of the truncated HMG-CoA reductase gene (tHMG1) has been shown to increase metabolic flux towards β-carotene production. Overexpression of tHMG1 was used to test the increase in overall production of carotenoids in our engineered strains of S. cerevisiae. All engineered strains were tested in a batch-mode bioreactor under pH, agitation, and temperature control.

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See more of this Session: Poster Session: Bioengineering
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division