386519 Continuous Production of Monoclonal Antibodies in Attached CHO CELL Culture MINI-Reactors: Use of Zein Foams As a Matrix for CELL Adhesion

Monday, November 17, 2014: 10:06 AM
206 (Hilton Atlanta)
Grissel trujillo De Santiago1, Sandra Ozuna Chacón2, Julián López-Meza2, Felipe López-Pacheco3, María del Rosario Rocha-Pizaña2, Diana Aráiz-Hernández2, Salvatore Iannace4, Ernesto Di Maio5 and Mario M. Alvarez6, (1)Centro de Biotecnología-FEMSA, Tecnoloógico de Monterrey, Monterrey, Mexico, (2)Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Monterrey, Nuevo León, Mexico, (3)Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Monterrey, N.L., Mexico, (4)Institute of Composite and Biomedical Materials, National Research Council of Italy, Portici, Naples, Italy, (5)Department of Chemical, Materials and Production Engineering, Faculty of Engineering, University of Naples Federico II, Naples, Italy, (6)Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Monterrey, Mexico

We present proof-of-principle results of the use of continuous mini-reactors (1mL scale) for the production of monoclonal antibodies in adherent CHO cell cultures using zein-foams as the scaffold for cell adhesion.  Zein foams were produced from thermo-plasticized zein exposed to supercritical CO2 foaming.

CHO cells were able to colonize and proliferate on the surface of the zein foams achieving confluence under continuous flow. Monoclonal antibody concentrations of 60 mg/L were achieved under steady state conditions at residence times of 1 hr. The maximum cell density of Chinese hamster ovary (CHO) cell cultures grown in the foam mini-bioreactors exceed 10 fold that observed in their stirred tank counterparts. Remarkably, their productivity is 100 fold higher with respect to the one observed in optimized fed-batch cultures in instrumented bioreactors (using the same cell line). Our results demonstrate that cell anchorage growth using plant-derived foamy surfaces is an attractive strategy for the production of biopharmaceutical compounds for screening and inclusively pilot plant scenarios.

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See more of this Session: Cell Culture I: Process and Media Design
See more of this Group/Topical: Food, Pharmaceutical & Bioengineering Division