382851 Spatial Patterning of BMP-2 and BMP-7 on Biopolymeric Films and the Guidance of Muscle Cell Fate

Monday, November 17, 2014: 9:24 AM
International 7 (Marriott Marquis Atlanta)
Jorge Almodovar1,2, Raphael Guillot2, Claire Monge2, Julien Vollaire3, Seila Selimovic4, Jean Luc-Coll3, Ali Khademhosseini5 and Catherine Picart2, (1)Department of Chemical Engineering, University of Puerto Rico Mayaguez, Mayaguez, PR, (2)Grenoble Institute of Technology, Grenoble, France, (3)Institute Albert Bonniot, Grenoble, France, (4)Department of Medicine, Harvard Medical School, Cambridge, MA, (5)Harvard-MIT, Division of Health Science and Technology, Massachusetts Institute of Technology, Cambridge, MA

In the cellular microenvironment, growth factor gradients are crucial in dictating cell fate. Towards developing materials that capture the native microenvironment we engineered biomimetic films that present gradients of matrix-bound bone morphogenetic proteins (BMP-2 and BMP-7). To this end layer-
by-layer films composed of poly(L-lysine) and hyaluronan were combined in a simple microfluidic device  enabling spatially controlled growth factor diffusion along the film. Linear long-range gradients of both  BMPs induced the trans-differentiation of C2C12 myoblasts towards the osteogenic lineage in a dose dependent manner with a different signature for each BMP. The osteogenic marker alkaline phosphatase (ALP) increased in a linear manner for BMP-7 and non-linearly for BMP-2. Moreover, an increased expression of the myogenic marker troponin T was observed with decreasing matrix-bound BMP concentration, providing a substrate that it is both osteo- and myo-inductive. Lastly, dual parallel matrix-bound gradients of BMP-2 and -7 revealed a complete saturation of the ALP signal. This suggested an additive or synergistic effect of the two BMPs. This simple technology allows for determining quickly and efficiently the optimal concentration of matrix-bound growth factors, as well as for investigating the presentation of multiple growth factors in their solid-phase and in a spatially controlled manner.

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See more of this Session: Biomaterials I
See more of this Group/Topical: Materials Engineering and Sciences Division