Preparation and Preliminary Antitumor Activity Evaluation of a New Targeted Nanoparticle System

PREPARATION AND PRELIMINARY ANTITUMOR ACTIVITY EVALUATION OF A NEW TARGETED NANOPARTICLE SYSTEM

, Eva M. Martin Del Valle*, José V. Román, Jesús A. Rodríguez-Rodríguez, Miguel A. Galán

Department of Chemical Engineering, University of Salamanca, Spain

*Corresponding author: emvalle@usal.es

Cancer is one of the main causes of human death. Most of usual antitumor treatments use chemotherapy agents and it involves damages in other organs because of their side effects.

In this respect, the aim of this work has been the design of a new targeted drug delivery nanoparticles system and its support with in vitro and in vivo tests for human lung cancer therapy.

The targeted nanoparticles were produced by electrostatic interactions under acid aqueous conditions. This nanoparticle was used to fix the antitumor drug and identification agent of tumoral cells on its surface.

The in vitro trials’ validation has been done by MTT assay in order to check the viability of tumor cells. Besides the in vivo effectiveness is controlled by animal experiments with mice. Mice tumors have been controlled though daily checks and subsequently analyzed by testing pathological anatomy.

Cell experiments have been developed with the H460 line cell and the effect of targeted drug nanoparticles displays a better behavior compared to the current chemotherapy.

Animal experiments present encouraging results for mice treated with nanoparticles and a longer life in these diseased animals compared to non-treated animals and even to animals treated with a conventional chemotherapy.

Therefore, the current study describes how manufacture an advanced targeted drug delivery nanoparticle system and its evaluation in vitro and in vivo experiments.